Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen.

نویسندگان

  • Parima Boontanom
  • Suradej Siripattanapipong
  • Mathirut Mungthin
  • Peerapan Tan-ariya
  • Saovanee Leelayoova
چکیده

A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).

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عنوان ژورنال:
  • The Southeast Asian journal of tropical medicine and public health

دوره 41 2  شماره 

صفحات  -

تاریخ انتشار 2010